New Publication

March 2020

High-resolution longitudinal N- and O-glycoprofiling of human monocyte-to- macrophage transition.
Protein glycosylation impacts the development and function of innate immune cells. The glyco-phenotypes and the glycan remodelling associated with the maturation of macrophages from monocytic precursor populations remain incompletely described. Herein, label-free PGC-LC-MS/MS was employed to profile with high resolution the N- and O-glycome associated with human monocyte-to-macrophage transition.
Check it out!

August 2019

Protein Paucimannosylation is an Enriched N‚Äźglycosylation Signature of Human Cancers.
This glycomics-centric study explored the association between protein paucimannosylation and a range of human cancer types enabled by access to almost 500 information-rich glycomics datasets collected over a decade using a uniform analytical strategy involving porous graphitised carbon-liquid chromatography-tandem mass spectrometry. This re-interrogation effort, the to-date largest of its kind in glycomics research, demonstrated that paucimannosidic N-glycans are enriched in human cancers.
Check it out!

Human protein paucimannosylation: cues from the eukaryotic kingdoms.
Protein paucimannosylation, an unusual truncated type of N-linked glycosylation of human proteins, was described almost four decades ago, but our knowledge of the structure, function and biosynthesis of this protein modification remains incomplete. With the aim to improve our understanding of the biological roles of paucimannosidic proteins in human glycobiology, we have comprehensively surveyed the literature describing protein paucimannosylation across the eukaryotic kingdoms. In short, this synthetic review finds that human paucimannosidic proteins are biological significant, yet still heavily under-studied biomolecules in human glycobiology that serve essential functions and create structural heterogeneity not dissimilar to other classes of human N-glycoproteins.
Check it out!

March 2019

Hinneburg H, Chatterjee S, Schirmeister F, Nguyen-Khuong T, Packer NH, Rapp E, Thaysen-Andersen M. Post-Column Make-up Flow (PCMF) Enhances the Performance of Capillary-Flow PGC-LC-MS/MS-Based Glycomics. Anal Chem2019. doi: 10.1021/acs.analchem.8b05720. [Epub ahead of print]

Back to the top of this page