Instrumentation

Instrumentation

APAF houses a range of instrumentation that is run by our team for service work. A subset of this instrumentation is available for training and access (denoted with an asterisk below), for more information please refer to our access guideline and contact the APAF team in person or at info.apaf@mq.edu.au

APAF is part of the Macquarie Analytical and Fabrication Facility (MAFF) within Macquarie University, for a range of other capabilities and instrumentation available within MAFF please visit the MAFF website, or email fse.maff-admin@mq.edu.au to get in contact with the team that can support your project needs.

APAF Instrumentation:

HPLC

  • Agilent 1200/1260/1290 Infinity series

UPLC

  • Acquity Class x 2
  • Acquity Premier Class
  • Acquity I-Class

Mass photometry

  • TwoMP Mass Photometer*

Mass spectrometry

Cyclic ion mobility select series MS (cIMS)*

  • Vendor: Waters
  • Capability: Cyclic ion mobility select series MS coupled with M-class LC system; flexible LC system capable of wide range of flow rates from nano- to micro-flow; cyclic IMS allows for resolution of PTMs on peptides and proteins, and varied fragmentation options allow for in-depth characterisation of proteoforms.

Orbitrap Eclipse (Coming Q1 2024)*

  • Vendor: ThermoFisher Scientific
  • Capability:

Orbitrap Exploris 480*

  • Vendor: ThermoFisher Scientific
  • Capability:

Q Exactive*

  • Vendor: ThermoFisher Scientific
  • Capability: QE classic orbitrap instrument coupled to nanoLC; ideal for bottom-up protein identification and qualitative proteomic analyses

Q Exactive Plus*

  • Vendor: ThermoFisher Scientific
  • Capability: QE+ orbitrap instrument coupled to Vanquish LC system; analytical flow rates on the Vanquish and high resolution of MS make this instrument suitable for small molecule and HRMS analysis.

Q Exactive HF-X*

  • Vendor: ThermoFisher Scientific
  • Capability: QE-HFX orbitrap MS coupled to a Dionex nanoLC; the high sensitivity and resolution of the HFX are ideal for quantitative proteomics experiments including label-free quantitation and TMT based experiments.

6600 Triple TOF (x 2)*

  • Vendor: ABSciex
  • Capability: 6600 tripleTOF coupled with Eksigent LC with either nano or micro flow capacity; TOF instrument suited to bottom-up proteomics and data-independent acquisition workflows.

Array technology

  • Bioplex 200*

Automation

  • Andrew+ Alliance Pipetting Robot

*Available for training and access

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