Reversed Phase - High Performance Liquid Chromatography (RP-HPLC)

APAF offers a variety of HPLC methods for separation and analysis of proteins and their fragments, based on their size, hydrophobicity and/or charge (Figure 1).

• Figure 1. Bulk separation methods available for sample preparation.
• APAF offers additional HPLC services such as high-pH fractionation and size-exclusion chromatography. HPLC is being used for separation or semi purification of proteins, monitoring batch to batch protein purification and identifying post-translational modifications of proteins in the field of molecular biology, genetics, microbiology, biochemistry and clinical chemistry.

Size exclusion chromatography (SEC)

• Size exclusion chromatography (SEC) is a mode of HPLC that employs porous particles to separate molecules in a solution by virtue of their size. SEC is generally used to separate biological molecules, to determine molecular weight distributions of proteins and peptides, and is used in a wide range of industries.

• Figure 2 – Example separation by SEC (669kDa ‐ 1.3kDa).
• Peptide SEC (13,100 Da – 250Da) is also available.
• Specialised methods are also available for the quantitation of the following analytes:
  • A1/A2 beta casein in milk
  • Lactoferrin analysis
  • Uric acid
• APAF also welcomes to opportunity for customised method development and collaboration.