Olympus FluoView FV 3000RS IX83 Inverted Confocal Microscope

Capabilities

• Imaging diverse fluorophores in fixed or live samples
• Temperature, humidity, CO₂ control
• Spectrally tunable detectors
• Ultra-fast resonant scanning (reduced photo-damage with live samples)
• 3D z-stacks, timelapse, multi-position timelapse

Microscope

• Olympus IX83 fully motorized inverted microscope
• Zero Drift Compensator (IX3-ZDC2) laser-based autofocus
• Ultrasonic automated stage and multiple area time lapse stage control software, as well as well-plate navigator tool for multi-area, time-lapse, and multi-area mosaic image stitching
• Tokai hit stage top, live-cell incubation chamber
• Analog and digital in-out box for synchronization with external devices

Tandem Scanners

• Conventional scanner for high-definition imaging
• Resonant scanner for high-speed imaging (capable of 30 FPS@512×512 and 438 FPS@512×32)

Light Sources

• All Diode Lasers with 4 Laser Lines–405 nm, 488 nm, 561 nm and 640 nm
• LED-based transmitted and epifluorescence illumination

Conventional Fluorescence Filters

• Dapi
• Green
• Red

Objectives

Air objectives:

• UPLSAPO10X2
• UCPLFLN20X,
• UPLSAPO40X2
• UAPON100XOTIRF; UAPON 100X TIRF, NA 1.49,

Silicone oil objectives:

• UPLSAPO30XS; UPLSAPO N 30X SI OIL, NA 1.05,WD 0.8MM, W/CC (with correction collar)
• UPLSAPO60XS; UPLSAPO N 60X SI OIL, NA 1.30, WD 0.3MM,W/CC

Nomarski Differential Interference contrast capability on 10x, 20x, 30x, 40x, 60x, 100x.

Computer and Software

• HP Windows 7 64bit Confocal Workstation and 30” Monitor
• Complete Fluoview Acquisition & Analysis Software provides FRAP, FRET, Ratiometric Imaging Display & Analysis, 3D Image Acquisition & Display, Live 16 Channel Spectral Unmixing, Fluorescence Intensity Measurements, Time-Lapse Imaging, and More…
• Olympus cellSens Dimension provided for additional post-acquisition data analysis and processing
• Includes Olympus cellSens Count & Measure Solution for threshold-based object detection as well as automatic object measurement and classification

Includes Olympus cellSens 3D Deconvolution Solution utilizing Constrained Iterative Deconvolution algorithm for improved resolution, contrast and dynamic range.

Detectors

• 4 High-Sensitivity Peltier-Cooled GaAsP Spectral Confocal Detectors with Olympus TruSpectral High-Efficiency Volume Phase Hologram spectral detection system (1-100 nm adjustable emission bandwidth with 2 nm spectral resolution)
• Transmitted light detector for Brightfield/DIC Image Acquisition