Spectroscopy and light scattering

Spectroscopy and light scattering

Obtaining structural information from the interaction between molecules and electromagnetic radiation

Spectrometer: Circular dichroism: Jasco J-1500

The J-1500 circular dichroism (CD) spectropolarimeter allows simultaneous measurement of 4 modes including CD, linear dichroism (LD), and absorbance. We have a range of accessories including long pass cut-off filters for excitation fluorescence spectroscopy, film stretchers for LD, Couette flow cells  for LD, multiple -position thermostatted cuvette holder. Users are generally expected to provide their own quartz cuvettes

Circular dichroism (CD) measures the difference in absorbance of left- and right- handed polarized light of chiral molecules, which is commonly used to analyse the conformation of macromolecules, particularly protein secondary structure.
Linear dichroism (LD) measures  the difference in absorption of light linearly polarised parallel and perpendicular to the orientation axis. LD can be used to give information about the conformation and orientation of structures with orientated molecules such as DNA complexes, fibrous proteins such as (e.g. cytoskeleton and amyloid  proteins) and membrane peptides/proteins in liposomes.
The J-1500 is a hybrid instrument consisting of a variable wavelength polarimeter for CD or LD and absorption spectrophotometer (~180-900 nm, Far-UV to Vis range). The J-1500 is fitted with a 6-position multi-cell changer and Peltier temperature control unit. This set-up is ideally suited to:

  • Determine protein secondary structure and analyse conformational changes
  • Nucleic acid structure determination and changes upon binding or melting
  • Other thermodynamic parameters and kinetics based measurements

Used for

Routine solution state experiments on chiral molecules with a range of more sophisticated experiments possible including temperature controlled, kinetics, linear dichroism, fluorescence detected CD and LD.

Specifications

  • Operates from 950 – 163 nm
  • Light source : 150 W air-cooled Xenon lamp or 450 W water-cooled Xenon lamp (factory-installed option)
  • Detector : Head-on photomultiplier tube PMT
  • Optional photomultiplier tube PMT
  • Optional InGaAs
  • Modulator : Piezoelastic modulator
  • Measurement wavelength range : 163 to 950 nm
  • Wavelength accuracy : 0.1 nm from 163 to 250 nm
  • 0.2 nm from 250 to 500 nm
  • 0.5 nm from 500 to 800 nm
  • Spectral bandwidth: 0.01 to 16 nm
  • Slit width : 1 to 4000 μm
  • Digital Integration Time (D.I.T.) : 0.1 msec to 30 sec.
  • Scanning mode : Continuous scan
    • Step scan
    • Auto response(D.I.T) scan
  • Scanning speed : up to 10000 nm/min

Contact

Alison Rodger or Sophie Goodchild
4 Wally’s walk, laboratory 302
Department of Molecular Sciences
Macquarie University
sophie.goodchild@mq.edu.au
T: 02 9850 8235

Rates

Instrument usage costs are summarized in our pricing document. Prices exclude consumables. Discounted rates apply for Macquarie university researchers.

Location

4 Wally’s walk, room 302 (restricted access)

Bookings

Facility homepage

Spectrometer: Circular dichroism: Jasco J-810

Circular dichroism (CD) measures the difference in absorbance of left- and right-handed polarized light, which can be a powerful tool for identification and characterisation of chiral molecules. The J-810 is a hybrid instrument consisting of a variable wavelength polarimeter and absorption  spectrophotometer (~180-900 nm, Far-UV to Vis range). Our J-810 is also fitted with an Optical Rotation Dispersion (ORD) module and Peltier temperature control unit making it ideally suited to measure optical purity/identification of small molecules and
natural products chemistry.

Specifications

  • Light source: 150 W Xenon arc lamp (air cooled)
  • Wavelength range: ~180 to ~900 nm (standard)
  • Four Channel Simultaneous Data Acquisition: Input channels: 2 internal plus 2 external signals can be acquired simultaneously
  • ORDM-401 unit fitted

Contact

Alison Rodger or Sophie Goodchild
4 Wally’s walk, laboratory 302
Department of Molecular Sciences
Macquarie University
sophie.goodchild@mq.edu.au
T: 02 9850 8235

Rates

Instrument usage costs are summarized in our pricing document. Prices exclude consumables. Discounted rates apply for Macquarie university researchers.

Location

4 Wally’s walk, room 302 (restricted access)

Bookings

Facility homepage

Spectrometer: Infra-red: Jasco FT/IR-4700

The FT/IR 4700 is a Mid-IR Fourier Transform Infrared (FTIR) spectrometer (wavenumber range 7800 to 350 cm-1) with high resolution (0.4cm-1 and S/N over 35,000:1), making it ideal for transmission measurements of solid, liquid and gas samples. A real-time data processing function can be used to observe and verify the spectrum during measurements.
The FT/IR 4700 can also be paired with the PIKE MIRacle™ Single Reflection ATR accessory with ZeSe crystal for high throughput attenuated total reflectance (ATR) measurements.

Specifications

  • Light source: High-intensity ceramic source
  • Wavenumber Range: 7,800 to 350 cm-1
  • Wavenumber accuracy: ± 0.01 cm-1 (theoretical value)
  • Maximum resolution: 0.4 cm-1
  • Optical System: Single beam
  • Sample chamber: Size: 200 mm (W) × 260 mm (D) × 185 mm (h) (can be nitrogen purged)

Contact

Alison Rodger or Sophie Goodchild
4 Wally’s walk, laboratory 302
Department of Molecular Sciences
Macquarie University
sophie.goodchild@mq.edu.au
T: 02 9850 8235

Rates

Instrument usage costs are summarized in our pricing document. Prices exclude consumables. Discounted rates apply for Macquarie university researchers.

Location

4 Wally’s walk, room 302 (restricted access)

Bookings

Facility homepage

Spectrometer: Light Scattering: Malvern Zeta Sizer Nano SP

The Malvern Zetasizer Nano is a light scattering system suitable for characterisation of colloid dispersions, nanoparticles and macromolecules such as polymer and protein solutions (3.8 nm to 100 μm, concentration ~0.1ppm to 40%w/v, pH 1-13, sample dependent).  Three  different light scattering measurements can be performed:

  • Dynamic Light Scattering (DLS) – is used to measure the diffusion of particles under Brownian motion, and convert this to a size distribution using the Stokes-Einstein relationship. Non-invasive back-scatter (NIBS) technology is incorporated to give better sensitivity and the ability
    to measure particles at high concentration.
  • Electrophoretic Light scattering (ELS) – is used to measure zeta potential. An electric field is applied to the sample and mixed mode measurement, phase analysis light scattering (M3-PALS) is used to measure electrophoretic mobility and determine a zeta potential distribution.
  • Static Light Scattering (SLS) – is used to determine the molecular weight (> ~1KDa) of proteins and polymers. The scattering intensity at a range of concentrations is measured to construct a Debye plot. From this the weight-average molecular weight and second virial coefficient can
    be calculated to provide a measure of protein solubility.

Specifications

  • Measurement range: 3.8nm – 100 microns diameter (sample dependent)
  • Measurement principle: Electrophoretic Light Scattering
  • Minimum sample volume: 150µL (20µL using diffusion barrier method)
  • Accuracy: 0.12µm.cm/V.s for aqueous systems using NIST SRM1980 standard reference material
  • Sensitivity: ~0.1-10mg/mL (BSA/Lysozyme)
  • Temperature control range: 0°C - 90°C +/-0.1
  • Light source: He-Ne laser 633nm, Max 4mW

Contact

Alison Rodger or Sophie Goodchild
4 Wally’s walk, laboratory 302
Department of Molecular Sciences
Macquarie University
sophie.goodchild@mq.edu.au
T: 02 9850 8235

Rates

Instrument usage costs are summarized in our pricing document. Prices exclude consumables. Discounted rates apply for Macquarie university researchers.

Location

4 Wally’s walk, room 302 (restricted access)

Bookings

Facility homepage

Spectrometer: Circular dichroism: Jasco J-1500

The J-1500 circular dichroism (CD) spectropolarimeter allows simultaneous measurement of 4 modes including CD, linear dichroism (LD), and absorbance. We have a range of accessories including long pass cut-off filters for excitation fluorescence spectroscopy, film stretchers for LD, Couette flow cells  for LD, multiple -position thermostatted cuvette holder. Users are generally expected to provide their own quartz cuvettes

Circular dichroism (CD) measures the difference in absorbance of left- and right- handed polarized light of chiral molecules, which is commonly used to analyse the conformation of macromolecules, particularly protein secondary structure.
Linear dichroism (LD) measures  the difference in absorption of light linearly polarised parallel and perpendicular to the orientation axis. LD can be used to give information about the conformation and orientation of structures with orientated molecules such as DNA complexes, fibrous proteins such as (e.g. cytoskeleton and amyloid
proteins) and membrane peptides/proteins in liposomes.

The J-1500 is a hybrid instrument consisting of a variable wavelength polarimeter for CD or LD and absorption spectrophotometer (~180-900 nm, Far-UV to Vis range). The J-1500 is fitted with a 6-position multi-cell changer
and Peltier temperature control unit. This set-up is ideally suited to:

  • Determine protein secondary structure and analyse conformational changes
  • Nucleic acid structure determination and changes upon binding or melting
  • Other thermodynamic parameters and kinetics based measurements

Used for

Routine solution state experiments on chiral molecules with a range of more sophisticated experiments possible including temperature controlled, kinetics, linear dichroism, fluorescence detected CD and LD.

Specifications

  • Operates from 950 – 163 nm
  • Light source : 150 W air-cooled Xenon lamp or 450 W water-cooled Xenon lamp (factory-installed option)
  • Detector : Head-on photomultiplier tube PMT
  • Optional photomultiplier tube PMT
  • Optional InGaAs
  • Modulator : Piezoelastic modulator
  • Measurement wavelength range : 163 to 950 nm
  • Wavelength accuracy : 0.1 nm from 163 to 250 nm
  • 0.2 nm from 250 to 500 nm
  • 0.5 nm from 500 to 800 nm
  • Spectral bandwidth: 0.01 to 16 nm
  • Slit width : 1 to 4000 μm
  • Digital Integration Time (D.I.T.) : 0.1 msec to 30 sec.
  • Scanning mode : Continuous scan
    • Step scan
    • Auto response(D.I.T) scan
  • Scanning speed : up to 10000 nm/min

Contact

Alison Rodger or Sophie Goodchild
4 Wally’s walk, laboratory 302
Department of Molecular Sciences
Macquarie University
sophie.goodchild@mq.edu.au
T: 02 9850 8235

Rates

Instrument usage costs are summarized in our pricing document. Prices exclude consumables. Discounted rates apply for Macquarie university researchers.

Location

4 Wally’s walk, room 302 (restricted access)

Bookings

Facility homepage

Spectrometer: Circular dichroism: Jasco J-810

Circular dichroism (CD) measures the difference in absorbance of left- and right-handed polarized light, which can be a powerful tool for identification and characterisation of chiral molecules. The J-810 is a hybrid instrument consisting of a variable wavelength polarimeter and absorption  spectrophotometer (~180-900 nm, Far-UV to Vis range). Our J-810 is also fitted with an Optical Rotation Dispersion (ORD) module and Peltier temperature control unit making it ideally suited to measure optical purity/identification of small molecules and  natural products chemistry.

Specifications

  • Light source: 150 W Xenon arc lamp (air cooled)
  • Wavelength range: ~180 to ~900 nm (standard)
  • Four Channel Simultaneous Data Acquisition: Input channels: 2 internal plus 2 external signals can be acquired simultaneously
  • ORDM-401 unit fitted

Contact

Alison Rodger or Sophie Goodchild
4 Wally’s walk, laboratory 302
Department of Molecular Sciences
Macquarie University
sophie.goodchild@mq.edu.au
T: 02 9850 8235

Rates

Instrument usage costs are summarized in our pricing document. Prices exclude consumables. Discounted rates apply for Macquarie university researchers.

Location

4 Wally’s walk, room 302 (restricted access)

Bookings

Facility homepage

Spectrometer: Light Scattering: Malvern Zeta Sizer Nano SP

The Malvern Zetasizer Nano is a light scattering system suitable for characterisation of colloid dispersions, nanoparticles and macromolecules such as polymer and protein solutions (3.8 nm to 100 μm, concentration ~0.1ppm to 40%w/v, pH 1-13, sample dependent).  Three  different light scattering measurements can be performed:

  • Dynamic Light Scattering (DLS) – is used to measure the diffusion of particles under Brownian motion, and convert this to a size distribution using the Stokes-Einstein relationship. Non-invasive back-scatter (NIBS) technology is incorporated to give better sensitivity and the ability
    to measure particles at high concentration.
  • Electrophoretic Light scattering (ELS) – is used to measure zeta potential. An electric field is applied to the sample and mixed mode measurement, phase analysis light scattering (M3-PALS) is used to measure electrophoretic mobility and determine a zeta potential distribution.
  • Static Light Scattering (SLS) – is used to determine the molecular weight (> ~1KDa) of proteins and polymers. The scattering intensity at a range of concentrations is measured to construct a Debye plot. From this the weight-average molecular weight and second virial coefficient can
    be calculated to provide a measure of protein solubility.

Specifications

  • Measurement range: 3.8nm – 100 microns diameter (sample dependent)
  • Measurement principle: Electrophoretic Light Scattering
  • Minimum sample volume: 150µL (20µL using diffusion barrier method)
  • Accuracy: 0.12µm.cm/V.s for aqueous systems using NIST SRM1980 standard reference material
  • Sensitivity: ~0.1-10mg/mL (BSA/Lysozyme)
  • Temperature control range: 0°C - 90°C +/-0.1
  • Light source: He-Ne laser 633nm, Max 4mW

Contact

Alison Rodger or Sophie Goodchild
4 Wally’s walk, laboratory 302
Department of Molecular Sciences
Macquarie University
sophie.goodchild@mq.edu.au
T: 02 9850 8235

Rates

Instrument usage costs are summarized in our pricing document. Prices exclude consumables. Discounted rates apply for Macquarie university researchers.

Location

4 Wally’s walk, room 302 (restricted access)

Bookings

Facility homepage

Spectrometer: Microwave Plasma-Atomic Emission Spectrometer: Agilent 4100 MP-AES

Microwave induced Plasma Atomic Emission Spectrophotometer (MP-AES) measures levels of  elements in solution.

Used for

Simultaneous multi-analyte determination of major and minor elements. Working range of low ppm to weight percent (wt. %).

Specifications

  • Nitrogen supplied
  • Samples must be digested in low concentrated nitric acid
  • A wide range of ICP grade standards available in teaching lab
  • Similar to ICP-MS, AAS.

Contact

Remi Rouquette
4 Wally’s walk, room 221
Department of Molecular Sciences
Macquarie University
remi.rouquette@mq.edu.au
T: 02 9850 4219

Rates

Instrument usage costs are summarized in our pricing document. Prices exclude consumables. Discounted rates apply for Macquarie university researchers.

Location

14 Sir Christopher Ondaatje Avenue, room 321.

Bookings please contact Dr Remi Rouquette

Facility homepage

Spectrometer: Nuclear Magnetic Resonance: Bruker AVIIIHD 400 MHz

The Avance 400 MHz NMR spectrometer, with the SampleXpress 60 Holder Autosampler and Iconnmr automation software, is the workhorse of the NMR facility. Samples are conveniently submitted and queued for analysis.

Bruker AVIIIHD 400 MHz NMR Spectrometer

Used for

Routine solution state 1D and 2D experiments on small molecules

Specifications

  • 5mm BBFO SmartProbe™ (19F/31P-109Ag)
  • Actively-shielded high performance Z-gradient
  • 19F{1H} and 2D 1H/19F correlation spectroscopy
  • Operating temperature range: -150°C to +150°C
  • Ascend 400 MHz (9.4 T) Magnet
  • Drift rate < 4.0 Hz/hr
  • BCUII (Bruker Chiller Unit)
  • Accessible temperatures ≥ 200 K
  • SampleXpress 60 Holder Autosampler

Contact

Nicole Cordina
4 Wally’s walk, room 221
Department of Molecular Sciences
Macquarie University
nicole.cordina@mq.edu.au
T: 02 9850 6916

Rates

Instrument usage costs are summarized in our pricing document. Prices exclude consumables. Discounted rates apply for Macquarie university researchers.

Location

4 Wally’s walk, room 014 (highly restricted access)

Bookings

Facility homepage

Spectrometer: Nuclear Magnetic Resonance: Bruker AVIIIHD 500 MHz

The Avance 500 MHz NMR spectrometer is set up to run variable temperature experiments (-70°C to 100°C) routinely. Very low temperature experiments (-70°C to -150°C) can also be run with use of the liquid nitrogen evaporator and staff assistance. It is highly sensitive for carbon detection, thus it is  often utilized to acquire data for the complete characterization of small to medium sized organic molecules.

Bruker AVIIIHD 500 MHz NMR Spectrometer

Used for

Variable temperature solution state experiments, characterization experiments

Specifications

  • 5mm BBFO SmartProbe™ (19F/31P-109Ag)
  • Actively-shielded high performance Z-gradient
  • 19F{1H} and 2D 1H/19F correlation spectroscopy
  • Operating temperature range: -150°C to +150°C
  • Ascend 500 MHz (11.7 T) Magnet
  • Drift rate < 5.0 Hz/hr
  • Magnet leg upgrade to damp vibrations above 3.8 Hz
  • BCUII (Bruker Chiller Unit)
  • Accessible temperatures ≥ 200 K
  • SampleCase 24 Holder Autosampler

Contact

Nicole Cordina
4 Wally’s walk, room 221
Department of Molecular Sciences
Macquarie University
nicole.cordina@mq.edu.au
T: 02 9850 6916

Rates

Instrument usage costs are summarized in our pricing document. Prices exclude consumables. Discounted rates apply for Macquarie university researchers.

Location

4 Wally’s walk, room 014 (highly restricted access)

Bookings

Facility homepage

Spectrometer: Nuclear Magnetic Resonance: Bruker AVII 600 MHz NMR Spectrometer

The AVII 600 MHz NMR spectrometer is equipped with cryoprobe and is utilized for experiments where the upmost of sensitivity is required, such as protein studies and natural product characterisations.

Bruker AVIIIHD 600 MHz NMR Spectrometer

Used for

Characterisation of natural products, protein NMR structure determination. Variable temperature work is not permitted.

Specifications

  • 5 mm TXI Cryoprobe with Z-gradient (1H/13C/15N/2H observe)
  • 3 high power channel operation
  • SampleXpress Lite 16 Holder Autosampler
  • BCU05 (Bruker Chiller Unit)

Contact

Nicole Cordina
4 Wally’s walk, room 221
Department of Molecular Sciences
Macquarie University
nicole.cordina@mq.edu.au
T: 02 9850 6916

Rates

Instrument usage costs are summarized in our pricing document. Prices exclude consumables. Discounted rates apply for Macquarie university researchers..

Location

4 Wally’s walk, room 014 (highly restricted access)

Bookings

Facility homepage

Spectrometer: Raman

Used for

High Precision Isotope Ratios

Contact

Bruce Schaefer
Bruce.Schaefer@mq.edu.au
T: 02 9850 8368

Location

E5B - 10 Sir Christopher Ondaatje Avenue, Room 135

Spectrometer: Spectrofluorometer: Jasco FP-8500

The FP-8500 is a spectrofluorometer for measurement of steady-state fluorescence and luminescence, emission and excitation, across a wavelength range of 200 to 750 nm. The FP-8500 has high resolution and standard automatic band-pass filters to obtain spectra without the artificial peaks due to second order diffracted light.
The FP-8500 is also fitted with shutters to protect samples when not being measured and rejection filters to minimise stray light. A Peltier temperature control unit is also available for temperature controlled measurements, making this instrument ideally suited for kinetics and thermodynamics  based experiments.

Specifications

  • Light Source: High intensity continuous output Xenon arc lamp
  • Wavelength range: 200 to 750 nm
  • Sensitivity: S/N 75,000:1 (RMS, water Raman)
  • Scanning Speed: 10-60,000 nm/min

Contact

Alison Rodger or Sophie Goodchild
4 Wally’s walk, laboratory 302
Department of Molecular Sciences
Macquarie University
sophie.goodchild@mq.edu.au
T: 02 9850 8235

Rates

Instrument usage costs are summarized in our pricing document. Prices exclude consumables. Discounted rates apply for Macquarie university researchers.

Location

4 Wally’s walk, room 302 (restricted access)

Bookings

Facility homepage

Spectrometer: UV-visible : Jasco V-760

The V-760 is a double-beam UV-Visible spectrometer that measures light absorption, reflectance or transmittance in the visible to ultraviolet region (187-900 nm).  The extended photometric linearity of the V-760 (up to 6 AU) enables measurement of highly absorbing  samples which is particularly useful for materials with low transmission properties such as solid films. The V-760 is also fitted with a 6-position multi-cell changer and Peltier temperature control unit making it an ideal UV-vis instrument for kinetic and thermodynamic  experiments.

Specifications

  • Optical System: Czerny-Turner mount, double monochromator, fully symmetrical double beam
  • Light Source: Halogen lamp, deuterium lamp
  • Wavelength range: 187 to 900 nm (+/-0.1 nm at 656.1 nm)
  • Photometric Range: -4-6 AU +/-0.3 %T
  • Scanning Speed: 10-400 nm/min

Contact

Alison Rodger or Sophie Goodchild
4 Wally’s walk, laboratory 302
Department of Molecular Sciences
Macquarie University
sophie.goodchild@mq.edu.au
T: 02 9850 8235

Rates

Instrument usage costs are summarized in our pricing document. Prices exclude consumables. Discounted rates apply for Macquarie university researchers.

Location

4 Wally’s walk, room 302 (restricted access)

Bookings

Facility homepage

Spectrometer: X-ray fluorescence: Bruker M4 Tornado Micro XRF

Used for

In-situ major element composition and elemental distribution of whole samples

Specifications

  • Typical Sample Type: Pretty much anything that will fit inside and withstand vacuum
  • Chamber size: 600mm x 360mm x 260mm
  • Resolution down to 20 μm

Contact

Timothy Murphy
E5B - 10 Sir Christopher Ondaatje Avenue, Room 204
Timothy.D.Murphy@mq.edu.au
T: 02 9850 8258

Location

E5B - 10 Sir Christopher Ondaatje Avenue, Room 206

Spectrometer: X-ray fluorescence: Panalytical Axios XRF

Used for

Major element analysis of fused glass beads produced from rock powders

Specifications

  • Typical Sample Type: 1 gram of <63 μm rock powder
  • Fused glass beads produced in-house using your sample
  • 1 kW Source

Contact

Peter Wieland
E5B - 10 Sir Christopher Ondaatje Avenue, Room 206
Peter.Wieland@mq.edu.au
T: 02 9850 4402

Location

E5B - 10 Sir Christopher Ondaatje Avenue, Room 206

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