Chemical purification and analysis

Chemical purification and analysis

For the isolation and physical/chemical analysis of a wide range of samples

Chromatography: HPLC-Agilent 1260 (x2)

The reversed phase HPLC is an instrument used for the separation, identification and quantification of various components in a mixture via UV-vis detection.

Used for:

  • Qualitative analysis
  • Quantitative analysis
  • Sample purification and separation

Specifications

  • Quaternary pump (flow range 0.001-10mL/min)
  • Autosampler (Injection volume 0.1-100 uL, 120 positions sample tray)
  • Fraction collector
  • Mobile phase: Water, Acetonitrile or Methanol (HPLC grade provided by user)
  • C18 columns available if needed

Contact

Remi Rouquette
4 Wally’s walk, room 221
Department of Molecular Sciences
Macquarie University
remi.rouquette@mq.edu.au
T: 02 9850 4219

Rates

Instrument costs are summarized in our pricing document. Prices exclude consumables eg solvents and columns. Discounted rates apply for Macquarie university researchers.

Location

4 Wally’s walk, room 309

Bookings – only after induction and training

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Chromatography: HPLC-Agilent 1290

The reversed phase HPLC is an instrument used for the separation, identification and quantification of various components in a mixture via UV, fluorescence, RID and ELSD detection.

Used for:

  • Qualitative analysis
  • Quantitative analysis
  • Sample purification and separation

Specifications

  • Agilent 385 ELSD
  • Fraction Collector analytical scale
  • Infinity Diode-Array Detector
  • Standard Autosampler
  • Infinity Autosampler
  • Infinity Binary Pump
  • Thermostatted Column Compartment
  • Infinity Fluorescence DetectorMobile phase: Water, Acetonitrile or Methanol (HPLC grade provided by user)

Contact

Remi Rouquette
4 Wally’s walk, room 221
Department of Molecular Sciences
Macquarie University
remi.rouquette@mq.edu.au
T: 02 9850 4219

Rates

Instrument costs are summarized in our pricing document. Prices exclude consumables eg solvents and columns. Discounted rates apply for Macquarie university researchers.

Location

14 Eastern road, room 220 (PC2 lab)

Bookings – only after induction and training

Facility homepage

Chromatography: GCMS-Shimadzu QP2010

Gas chromatogram mass spectrometer is an instrument used for the separation, identification and quantification of various components in a mixture via flame ionization and mass detection.

Used for:

  • Qualitative analysis
  • Quantitative analysis
  • Mass analysis
  • Only volatile molecules will be detected

Specifications

  • Injection mode: Direct, Autosampler (120 positions sample tray), SPME
  • Single quadrupole (SIM/scan mode, 10 to 1000 m/z, Electron ionization)
  • Flame ionization detector (FID)
  • Mobile phase: UHP Helium (column flow rate 10 mL/min)
  • Large selection of capillary columns available on demand

Contact

Remi Rouquette
4 Wally’s walk, room 221
Department of Molecular Sciences
Macquarie University
remi.rouquette@mq.edu.au
T: 02 9850 4219

Rates

Instrument costs are summarized in our pricing document. Prices exclude consumables eg solvents and columns. Discounted rates apply for Macquarie university researchers.

Location

4 Wally’s walk, room 202

Bookings – only after induction and training

Facility homepage

Chromatography: GC-MS/MS-Shimadzu TQ8040

Gas chromatogram mass spectrometer is an instrument used for the separation, identification and quantification of various components in a mixture via flame ionization and mass detection.

Used for:

  • Qualitative analysis
  • Quantitative analysis
  • Mass analysis
  • Only volatile molecules will be detected

Specifications

  • Injection mode: Direct, Autosampler (120 positions sample tray), SPME, Headspace
  • Triple quadrupole (SIM, scan, MRM mode, 10 to 1000 m/z, Electron ionization)
  • Flame ionization detector (FID)
  • Mobile phase: UHP Helium (column flow rate 10 mL/min)
  • Large selection of capillary columns available on demand

Contact

Remi Rouquette
4 Wally’s walk, room 221
Department of Molecular Sciences
Macquarie University
remi.rouquette@mq.edu.au
T: 02 9850 4219

Rates

Instrument costs are summarized in our pricing document. Prices exclude consumables eg solvents and columns. Discounted rates apply for Macquarie university researchers.

Location

4 Wally’s walk, room 202

Bookings – only after induction and training

Facility homepage

Chromatography: LC/MS-Agilent 1260

The reversed phase LC/MS is an instrument used for the separation, identification and quantification of various components in a mixture via UV-vis and mass detection.

Used for:

  • Qualitative analysis
  • Quantitative analysis
  • Mass analysis

Specifications

  • Quaternary pump (flow range 0.001-10mL/min)
  • Autosampler (Injection volume 0.1-100 L, 120 positions sample tray)
  • Single quadrupole (SIM/scan mode, positive and negative polarity, ESI-API ionization)
  • Diode Array Detector (190-640 nm)
  • Mobile phase: Water, Acetonitrile or Methanol + 0.1% formic acid (LC/MS grade provided by department)
  • C18 columns available if needed

Contact

Remi Rouquette
4 Wally’s walk, room 221
Department of Molecular Sciences
Macquarie University
remi.rouquette@mq.edu.au
T: 02 9850 4219

Rates

Instrument costs are summarized in our pricing document. Prices exclude consumables eg solvents and columns. Discounted rates apply for Macquarie university researchers.

Location

4 Wally’s walk, room 311

Bookings

Facility homepage

Elemental Analyser: Elementar®- Vario Micro Cube

Analysis of carbon, hydrogen, nitrogen and sulfur content for a wide range of samples.

Used for: C%, H%, N% and S% in sample

Specifications

  • For solid and liquid samples
  • Minimum amount of 2 mg per sample for duplicates.

Contact

Remi Rouquette
4 Wally’s walk, room 221
Department of Molecular Sciences
Macquarie University
remi.rouquette@mq.edu.au
T: 02 9850 4219

Rates

Instrument costs are summarized in our pricing document. Prices exclude consumables eg solvents and columns. Discounted rates apply for Macquarie university researchers.

Location

10 Sir Christopher Ondaatje Avenue, room 221

Facility homepage

Spectrometer: Circular dichroism: Jasco J-810

Circular dichroism (CD) measures the difference in absorbance of left- and right-handed polarized light, which can be a powerful tool for identification and characterisation of chiral molecules. The J-810 is a hybrid instrument consisting of a variable wavelength polarimeter and absorption  spectrophotometer (~180-900 nm, Far-UV to Vis range). Our J-810 is also fitted with an Optical Rotation Dispersion (ORD) module and Peltier temperature control unit making it ideally suited to measure optical purity/identification of small molecules and  natural products chemistry.

Specifications

  • Light source: 150 W Xenon arc lamp (air cooled)
  • Wavelength range: ~180 to ~900 nm (standard)
  • Four Channel Simultaneous Data Acquisition: Input channels: 2 internal plus 2 external signals can be acquired simultaneously
  • ORDM-401 unit fitted

Contact

Alison Rodger or Sophie Goodchild
4 Wally’s walk, laboratory 302
Department of Molecular Sciences
Macquarie University
sophie.goodchild@mq.edu.au
T: 02 9850 8235

Rates

Instrument usage costs are summarized in our pricing document. Prices exclude consumables. Discounted rates apply for Macquarie university researchers.

Location

4 Wally’s walk, room 302 (restricted access)

Bookings

Facility homepage

Spectrometer: Infra-red: Jasco FT/IR-4700

The FT/IR 4700 is a Mid-IR Fourier Transform Infrared (FTIR) spectrometer (wavenumber range 7800 to 350 cm-1) with high resolution (0.4cm-1 and S/N over 35,000:1), making it ideal for transmission measurements of solid, liquid and gas samples. A real-time data processing function can be used to observe and verify the spectrum during measurements.
The FT/IR 4700 can also be paired with the PIKE MIRacle™ Single Reflection ATR accessory with ZeSe crystal for high throughput attenuated total reflectance (ATR) measurements.

Specifications

  • Light source: High-intensity ceramic source
  • Wavenumber Range: 7,800 to 350 cm-1
  • Wavenumber accuracy: ± 0.01 cm-1 (theoretical value)
  • Maximum resolution: 0.4 cm-1
  • Optical System: Single beam
  • Sample chamber: Size: 200 mm (W) × 260 mm (D) × 185 mm (h) (can be nitrogen purged)

Contact

Alison Rodger or Sophie Goodchild
4 Wally’s walk, laboratory 302
Department of Molecular Sciences
Macquarie University
sophie.goodchild@mq.edu.au
T: 02 9850 8235

Rates

Instrument usage costs are summarized in our pricing document. Prices exclude consumables. Discounted rates apply for Macquarie university researchers.

Location

4 Wally’s walk, room 302 (restricted access)

Bookings

Facility homepage

Spectrometer: Microwave Plasma-Atomic Emission Spectrometer: Agilent 4100 MP-AES

Microwave induced Plasma Atomic Emission Spectrophotometer (MP-AES) measures levels of  elements in solution.

Used for: simultaneous multi-analyte determination of major and minor elements. Working range of low ppm to weight percent (wt. %).

Specifications

  • Nitrogen supplied
  • Samples must be digested in low concentrated nitric acid
  • A wide range of ICP grade standards available in teaching lab
  • Similar to ICP-MS, AAS.

Contact

Remi Rouquette
4 Wally’s walk, room 221
Department of Molecular Sciences
Macquarie University
remi.rouquette@mq.edu.au
T: 02 9850 4219

Rates

Instrument usage costs are summarized in our pricing document. Prices exclude consumables. Discounted rates apply for Macquarie university researchers.

Location

14 Sir Christopher Ondaatje Avenue, room 321.

Bookings please contact Dr Remi Rouquette

Facility homepage

Spectrometer: Nuclear Magnetic Resonance: Bruker AVIIIHD 400 MHz

The Avance 400 MHz NMR spectrometer, with the SampleXpress 60 Holder Autosampler and Iconnmr automation software, is the workhorse of the NMR facility. Samples are conveniently submitted and queued for analysis.

Bruker AVIIIHD 400 MHz NMR Spectrometer

Used for: routine solution state 1D and 2D experiments on small molecules.

Specifications

  • 5mm BBFO SmartProbe™ (19F/31P-109Ag)
  • Actively-shielded high performance Z-gradient
  • 19F{1H} and 2D 1H/19F correlation spectroscopy
  • Operating temperature range: -150°C to +150°C
  • Ascend 400 MHz (9.4 T) Magnet
  • Drift rate < 4.0 Hz/hr
  • BCUII (Bruker Chiller Unit)
  • Accessible temperatures ≥ 200 K
  • SampleXpress 60 Holder Autosampler

Contact

Nicole Cordina
4 Wally’s walk, room 221
Department of Molecular Sciences
Macquarie University
nicole.cordina@mq.edu.au
T: 02 9850 6916

Rates

Instrument usage costs are summarized in our pricing document. Prices exclude consumables. Discounted rates apply for Macquarie university researchers.

Location

4 Wally’s walk, room 014 (highly restricted access)

Bookings

Facility homepage

Spectrometer: Nuclear Magnetic Resonance: Bruker AVIIIHD 500 MHz

The Avance 500 MHz NMR spectrometer is set up to run variable temperature experiments (-70°C to 100°C) routinely. Very low temperature experiments (-70°C to -150°C) can also be run with use of the liquid nitrogen evaporator and staff assistance. It is highly sensitive for carbon detection, thus it is  often utilized to acquire data for the complete characterization of small to medium sized organic molecules.

Bruker AVIIIHD 500 MHz NMR Spectrometer

Used for: variable temperature solution state experiments, characterization experiments

Specifications

  • 5mm BBFO SmartProbe™ (19F/31P-109Ag)
  • Actively-shielded high performance Z-gradient
  • 19F{1H} and 2D 1H/19F correlation spectroscopy
  • Operating temperature range: -150°C to +150°C
  • Ascend 500 MHz (11.7 T) Magnet
  • Drift rate < 5.0 Hz/hr
  • Magnet leg upgrade to damp vibrations above 3.8 Hz
  • BCUII (Bruker Chiller Unit)
  • Accessible temperatures ≥ 200 K
  • SampleCase 24 Holder Autosampler

Contact

Nicole Cordina
4 Wally’s walk, room 221
Department of Molecular Sciences
Macquarie University
nicole.cordina@mq.edu.au
T: 02 9850 6916

Rates

Instrument usage costs are summarized in our pricing document. Prices exclude consumables. Discounted rates apply for Macquarie university researchers.

Location

4 Wally’s walk, room 014 (highly restricted access)

Bookings

Facility homepage

Spectrometer: Nuclear Magnetic Resonance: Bruker AVII 600 MHz NMR Spectrometer

The AVII 600 MHz NMR spectrometer is equipped with cryoprobe and is utilized for experiments where the upmost of sensitivity is required, such as protein studies and natural product characterisations.

Bruker AVIIIHD 600 MHz NMR Spectrometer

Used for: characterisation of natural products, protein NMR structure determination. Variable temperature work is not permitted.

Specifications

  • 5 mm TXI Cryoprobe with Z-gradient (1H/13C/15N/2H observe)
  • 3 high power channel operation
  • SampleXpress Lite 16 Holder Autosampler
  • BCU05 (Bruker Chiller Unit)

Contact

Nicole Cordina
4 Wally’s walk, room 221
Department of Molecular Sciences
Macquarie University
nicole.cordina@mq.edu.au
T: 02 9850 6916

Rates

Instrument usage costs are summarized in our pricing document. Prices exclude consumables. Discounted rates apply for Macquarie university researchers..

Location

4 Wally’s walk, room 014 (highly restricted access)

Bookings

Facility homepage

Spectrometer: UV-visible : Jasco V-760

The V-760 is a double-beam UV-Visible spectrometer that measures light absorption, reflectance or transmittance in the visible to ultraviolet region (187-900 nm).  The extended photometric linearity of the V-760 (up to 6 AU) enables measurement of highly absorbing  samples which is particularly useful for materials with low transmission properties such as solid films. The V-760 is also fitted with a 6-position multi-cell changer and Peltier temperature control unit making it an ideal UV-vis instrument for kinetic and thermodynamic  experiments.

Specifications

  • Optical System: Czerny-Turner mount, double monochromator, fully symmetrical double beam
  • Light Source: Halogen lamp, deuterium lamp
  • Wavelength range: 187 to 900 nm (+/-0.1 nm at 656.1 nm)
  • Photometric Range: -4-6 AU +/-0.3 %T
  • Scanning Speed: 10-400 nm/min

Contact

Alison Rodger or Sophie Goodchild
4 Wally’s walk, laboratory 302
Department of Molecular Sciences
Macquarie University
sophie.goodchild@mq.edu.au
T: 02 9850 8235

Rates

Instrument usage costs are summarized in our pricing document. Prices exclude consumables. Discounted rates apply for Macquarie university researchers.

Location

4 Wally’s walk, room 302 (restricted access)

Bookings

Facility homepage

Thermal analysis: TGA- TA instrument Thermal

Thermogravimetric analysis monitors the weight change of a wide range of samples upon variation of temperature

Used for: mass variation of sample under normal or inert atmosphere.

Specifications

  • Heating up to 1000°C
  • Aluminum or ceramic pans
  • Air or Nitrogen atmosphere

Contact

Remi Rouquette
4 Wally’s walk, room 221
Department of Molecular Sciences
Macquarie University
remi.rouquette@mq.edu.au
T: 02 9850 4219

Rates

Instrument usage costs are summarized in our pricing document. Prices exclude consumables. Discounted rates apply for Macquarie university researchers.

Location

4 Wally,s Walk, room 202.

Bookings

Facility homepage

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