Mass Spectrometry
Mass Spectrometry
Advances in mass spectrometry and data analysis have provided new opportunities to comprehensively investigate protein expression in complex biological systems. The Australian Proteomics Analysis Facility has been providing mass spectrometry services for the analysis of proteins and proteomes since 1995 and today we make use of highly sensitive and accurate quantitative MS instrumentation and platforms to comprehensive survey even the most complex of biological systems. We can assess alterations to key biological pathways which might be brought about by changes in disease state, condition, treatment or a stimulus. We currently use a range of state-of-the-art mass spectrometry platforms including Q-tof, Orbitrap and Q-trap technologies coupled to nano-LC and UHPLC systems to provide highly sensitive detection and quantitation capabilities. We provide access to this high-end LC-MS instrumentation and work closely with researchers in industry, academia and government organisations to support multidisciplinary projects which span the chemical and biological sciences, biomedicine and health, food and agriculture, and microbiology. APAF’s core MS-expertise is in quantitative proteomics and protein identification and characterisation. We also specialise in small molecule characterisation and quantitation.
| Instrument | Vendor | Capability |
|---|---|---|
| Fusion Lumos | ThermoFisher | Fusion Lumos coupled to a Dionex nanoLC; High resolution tribrid instrument with IT and OT; ExD collision cell; FAIMS source; static spray source. Static spray, tribrid system with ECD capability makes the instrument ideal for in depth characterisation of protein and PTMs and intact mass analysis. |
| 6600 Triple TOF | ABSciex | 6600 tripleTOF coupled with Eksigent LC with either nano or micro flow capacity; TOF instrument suited to bottom-up proteomics and data-independent acquisition workflows. |
| QE-HFX | ThermoFisher | QE-HFX orbitrap MS coupled to a Dionex nanoLC; the high sensitivity and resolution of the HFX are ideal for quantitative proteomics experiments including label-free quantitation and TMT based experiments. |
| QE+ | ThermoFisher | QE+ orbitrap instrument coupled to Vanquish LC system; analytical flow rates on the Vanquish and high resolution of MS make this instrument suitable for small molecule and HRMS analysis. |
| QE | ThermoFisher | QE classic orbitrap instrument coupled to nanoLC; ideal for bottom-up protein identification and qualitative proteomic analyses |
| cIMS | Waters | Cyclic ion mobility select series MS coupled with M-class LC system; flexible LC system capable of wide range of flow rates from nano- to micro-flow; cyclic IMS allows for resolution of PTMs on peptides and proteins, and varied fragmentation options allow for in-depth characterisation of proteoforms. |
Some recent examples include:
