Advances in mass spectrometry and data analysis have provided new opportunities to comprehensively investigate protein expression in complex biological systems. The Australian Proteomics Analysis Facility has been providing mass spectrometry services for the analysis of proteins and proteomes since 1995 and today we make use of highly sensitive and accurate quantitative MS instrumentation and platforms to comprehensive survey even the most complex of biological systems. We can assess alterations to key biological pathways which might be brought about by changes in disease state, condition, treatment or a stimulus. We currently use a range of state-of-the-art mass spectrometry platforms including Q-tof, Orbitrap and Q-trap technologies coupled to nano-LC and UHPLC systems to provide highly sensitive detection and quantitation capabilities. We provide access to this high-end LC-MS instrumentation and work closely with researchers in industry, academia and government organisations to support multidisciplinary projects which span the chemical and biological sciences, biomedicine and health, food and agriculture, and microbiology. APAF’s core MS-expertise is in quantitative proteomics and protein identification and characterisation. We also specialise in small molecule characterisation and quantitation.
Some recent examples include: