Gel electrophoresis (GE) is a method for separation and analysis of proteins and their fragments, based on their size and/or charge (Figure 1). APAF offers GE and Western blot services using either one-dimensional (1-D) or two-dimensional (2-D) SDS-PAGE and using the precast gels only. GE is being used for separation or semi purification of proteins, monitoring batch to batch protein purification, identifying post-translational modifications of proteins, and validation of putative biomarkers by Western blot analysis in the field of molecular biology, genetics, microbiology, biochemistry and clinical chemistry.
Figure 1. One-dimensional (1-D) separation of proteins on SDS-PAGE and Western Blot analysis.
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- Cristian, V., et al. Systems biology: a new paradigm for industrial yeast strain development. Microbiology Australia, 2011, 32: 151-155 (2011). DOI: 10.4172/2153-0769.S1.014.
- Polaskova, V., et al. High-abundance protein depletion: Comparison of methods for human plasma biomarker discovery. Electrophoresis 2010, 31:471-482. DOI: 10.1002/elps.200900286.
- Gibson, F., et al. MIPAE: Gel electrophoresis. Nature Biotechnology, 2008, 26: 863-864. DOI: 10.1038/nbt0808-863.
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