APAF Newsletter Vol. 7 No. 2

Welcome to APAF's second 2017 newsletter.

In this newsletter we showcase APAF capabilities with SWATH-MS and Luminex multiplexed immunoassays. In a report recently published in Nature Communications, APAF’s use of SWATH-MS was compared with 10 international proteomic facilitates demonstrating our world leading excellence in applying this important proteomic technique. We report use of SWATH-MS in a collaboration with researchers at the Murdoch Childrens Research Institute to investigate the paediatric plasma proteome. Keeping with the theme of aging, but on a different note, we showcase a study linking frailty in older men with inflammatory markers which we measured using multiplexed immunoassay with researchers at the ANZAC Institute.

APAF showcases it's SWATH-MS capabilities

In a study published in Nature Communications, a multi-laboratory international assessment of SWATH mass spectrometry (also known as data-independent acquisition, or DIA) confirms excellent reproducibility and quantitative performance this this technique for proteomics. The Australian Proteome Analysis Facility (APAF) was selected as the only Australian participant in this study of 11 international laboratories and confirms APAF’s superiority to reliably detect and quantitate peptides from complex human cell lysates using SWATH-MS. APAF’s median intra-day and inter-day CV for quantitating 4077 proteins was ~7% and ~9%, respectively, values that were lower than the medians reported for the entire 11 laboratories. Read more

Revealing the secrets of the paediatric plasma proteome with SWATH-MS

The human plasma proteome is of great interest for biomedical research as a potential source of protein biomarkers. Most studies have examined plasma from healthy and diseased adults while analysis of paediatric plasma is very limited. In collaboration with Dr Vera Ignjatovic and colleagues from Murdoch Childrens Research Institute we recently measured the paediatric plasma proteome using SWATH-MS. Read more

Relationship between inflammatory Biomarkers and Frailty

Inflammation underlies many diseases and previous research supports a role for some inflammatory markers to be associated with frailty. In a recent study with Prof David Le Couteur and colleagues from the ANZAC Institute, APAF conducted a large-scale screen using multiplexed immunoassays to quantitate 27 cytokines and chemokines from plasma of older men (>70years) in the CHAMP study “Concord Health and Ageing in Men Project”. Read more