Mass spectrometry analysis of intact protein
The intact protein is analysed by either ESI-TOF MS or Linear MALDI-TOF MS. Generally, ESI-TOF MS is recommended as it is more accurate and requires less sample. Proteins are treated in a variety of ways depending on the sample and the MS method used. Once analysed the result is reported as a mass, plus or minus the experimental error.
The accurate assignment of a proteins mass
Highly accurate mass information (accurate to 0.01% for ESI-TOF)
Can be used for basic glycoprotein analysis
Generally larger amounts of sample are required
The protein must be pure (>90%)
Ideally the sample is in solution, or solid format
Not all proteins are amenable to MS analysis
Highly glycosylated, or variably glycosylated, proteins can be difficult to analyse
For further details or advice on this APAF service please contact us at firstname.lastname@example.org.